Semantic Coherence: PacBio – Signal Evidence & AI Readability

Revio system   Order now

[H3]
Your guide to getting started
Have a sequencing question, application, or project in mind? There’s HiFi for that. Download the newest HiFi Starter Kit to explore where long-read sequencing fits and how to take the next step.
Get the kit

[H3]
Expert Panel: Implementation of HiFi sequencing in clinical research laboratories
In this expert panel, hear how leading teams are evaluating HiFi WGS and PureTarget across rare disease, carrier screening, and broader human genomics workflows, with practical insight on assay consolidation, evaluation, adoption, and scale.
Register now

[H3]
Join us at ASM Microbe 2026 - June 4-7
Visit us at booth #1325 to meet PacBio experts, see live demonstrations of our Vega and Revio sequencing systems, and learn how HiFi sequencing can empower your genetic testing workflows. Sign up to receive PacBio posters, presentations, and additional resources from the conference.
Access event content

[H2]
Sequencing methods for solutions that matter

[IMG: Whole genome sequencing stylized icon representing whole genome sequencing]

[H3]
Whole genome sequencing
Achieve the highest consensus accuracy and uniform coverage for reference quality genomes in humans, plants, animals, and microbes.

Learn more

[IMG: Targeted sequencing Icon representing targeted sequencing]

[H3]
Targeted sequencing
Accurately detect and uncover all variant types even in hard-to-reach regions of the genome.

Learn more

[IMG: Color epigenetics icon]

[H3]
Epigenetics
Explore how epigenetic changes affect gene expression, host-pathogen interactions, environmental response, and more.

Learn more

[IMG: RNA sequencing Icon representing RNA]

[H3]
RNA sequencing
Discover new genes, transcripts, and alternative splicing events with a complete view of transcript isoform diversity.

Learn more

[H2]
Accelerate your research

[IMG: Color human genomics icon]

Human genomics

[IMG: Color microbial sequencing icon]

Microbial genomics

[IMG: Color Plant + animal icon of a cow and leaf]

Plant + animal genomics

[IMG: Color cancer genomics research icon]

Cancer genomics

[IMG: Color crispr gene editing icon]

Gene therapy

[H2]

Application note

[H2]
Kinnex full-length RNA Kit for isoform sequencing

The Kinnex full-length RNA kit for bulk RNA sequencing produces 15 million reads and 40 million reads per SMRT Cell on Sequel II and Revio systems. This represents a 15-fold throughput increase on Revio with Kinnex.

See how
[IMG: Kinnex emblem]

[IMG: Target enrichment image]

A roadmap to expand PureTarget repeat expansion and carrier panels, enabling high-throughput clinical research solutions

Introducing two new panels – an updated repeat expansion panel and a new carrier screening panel – plus a 96-plex automation kit and a full software pipeline for carrier screening. Together, these tools streamline legacy assays into one efficient, high-throughput workflow to provide more value for key applications in clinical and diagnostic research and carrier screenin

Learn more

Sequencing 101: Long-read sequencing

Long-read sequencing technologies are quickly becoming the new gold standard in genomics research, and HiFi sequencing is at the forefront. This article provides an introductory look at what long-read sequencing is, and explores topics the advantages, applications of using long-read sequencing in your research.

Learn more

[IMG: Image of a PacBio SMRT Cell over a blue background]

What can you do with a Revio system?

Access more information at reduced cost and higher throughput with Revio long-read sequencing.

See what you can get

[IMG: Sprite decoration]

Explore

10,000+ articles, reports, papers, and videos

Learn more

[H2]
Recent press
New item found.

PacBio SPRQ-Nx Chemistry Now Shipping Worldwide, Enabling Sub-$300 HiFi Genomes for Large Scale Projects and AI-Enhanced Sequencing
May 26, 2026 | GLOBE NEWSWIRE

PacBio to Participate in the Jefferies Global Healthcare Conference
May 21, 2026 | GLOBE NEWSWIRE

HiFi Solves Sub-fertility Consortium in Asia Pacific Reports First Major Study Using HiFi Long-Read Sequencing to Investigate Unexplained Subfertility and Recurrent Pregnancy Loss
May 11, 2026 | GLOBE NEWSWIRE

View all
[H2]
Recent articles

The pivotal decisions that make for great sequencing data
May 28, 2026

Publication spotlight: How one HiFi sequencing assay can bring clarity to unexplained infertility
May 21, 2026

The power of one: How assay consolidation and menu expansion reshape sequencing economics
May 14, 2026

View all

[H1] Global Distributors
PacBio partners with leading distributors around the globe. Czech Republic, Slovakia, Hungary and Poland
[H3] 3Genes
PacBio Products Distributed: All Email: [email protected] Middle East & North Africa (MAGREB)
[H3] Alliance Global (AGBL)
PacBio Products Distributed: All Algeria, Bahrain, Egypt, Iraq, Jordon, Kuwait, Lebanon, Libya, Morocco, Oman, Qatar, Saudi Arabia, Tunisia, UAE and Uzbekistan Email: [email protected] Beijing China
[H3] Beijing Emei Tongde Technology Development Co., Ltd
PacBio Products Distributed: All Email: [email protected] Peru
[H3] Belomed
PacBio Products Distributed: All Email: [email protected] Greece
[H3] Biodynamics, S. A.
PacBio Products Distributed: All Email: [email protected] TAIWAN
[H3] Blossom Biotech
PacBio Products Distributed: All Email: [email protected] New Zealand
[H3] Custom Science
PacBio Products Distributed: All Email: [email protected] Estonia, Latvia and Lithuania
[H3] Diamedica
PacBio Products Distributed: All Websites by country: Estonia: https://www.diamedica.ee/et/kontakt Lithuania: https://www.diamedica.lt/ Latvia: https://www.diamedica.lv/lv/sia-diamedica-kontakti Estonia Email: [email protected]Lithuania Email: [email protected]Latvia Email: [email protected] Australia
[H3] DKSH BioStrategy
PacBio Products Distributed: All Email - ID: [email protected] SERBIA, MONTENEGRO, BOSNA AND HERZEGOVINA, MACEDONIA, CROATIA, KOSOVO, ROMANIA, BULGARIA, SLOVENIA AND ALBANIA
[H3] East Diagnostics
PacBio Products Distributed: All Bulgaria, Croatia, Herzegovina, Slovenia, Albania, Kosovo, Montenegro, North Macedonia, Romania, Bosnia, Serbia Email: [email protected] Israel
[H3] Eisenberg Bros. Ltd.
PacBio Products Distributed: All Email: [email protected] Turkey
[H3] EYS Medikal & Lab. Pro. Ltd.
PacBio Products Distributed: All Email: [email protected] China, Hong Kong and Macau
[H3] Gene Company Limitied
PacBio Products Distributed: All Email: [email protected] USA
[H3] Government Scientific Source
PacBio Products Distributed: All USA (federal gov’t entities only) Email: [email protected] Hangzhou，China
[H3] Hangzhou Baocheng Biotech Co., Ltd
PacBio Products Distributed: All Email: [email protected] Bangladesh
[H3] Hi-Tech Industrial Technologies / Interscience
PacBio Products Distributed: All Email: [email protected] Sub-Saharan Africa
[H3] Inqaba Biotec
PacBio Products Distributed: All Southern Africa: [email protected]East Africa: [email protected]West Africa: [email protected] Pakistan
[H3] JB Scientific
PacBio Products Distributed: All Email: [email protected] Republic of Korea
[H3] MDXK (Molecular Diagnostics Korea), Inc.
PacBio Products Distributed: All A- 801, D-tech Tower, 33, Gwacheon-daero 7-gil, Gwacheon-si, Gyeonggi-do, Republic of Korea, 13840 Email: [email protected] Japan
[H3] Nippon Genetics Co., Ltd
PacBio Products Distributed: Nanobind Email: [email protected] Brazil
[H3] PensaBio
PacBio Products Distributed: All Email: [email protected] Panama
[H3] Rochem Biocare de Panamá S.A.
PacBio Products Distributed: All Email: [email protected] Portugal
[H3] Soquimica
PacBio Products Distributed: All Email: [email protected] India
[H3] Spinco Biotech
PacBio Products Distributed: All Bangladesh, India, Nepal, Sri Lanka Email: [email protected] Italy
[H3] Tema Ricerca
PacBio Products Distributed: All Email: [email protected] Japan
[H3] Tomy Digital Biology Co., Ltd.
PacBio Products Distributed: All Email: [email protected] Ecuador
[H3] WAFIQ CIA LTDA
PacBio Products Distributed: All Email: [email protected] Shandong (China)
[H3] Wisdom Spring Jinan Biotech Co., Ltd.
PacBio Products Distributed: All Email: [email protected] Hubei (China)
[H3] Wuhan Naosaisi Instrument Co., Ltd.
PacBio Products Distributed: All Email: [email protected] Xi’an China
[H3] Xi’An Haorui Gene Technologies Ltd.
PacBio Products Distributed: All Email: [email protected]
[H6] Search
[H6] Region
Clear filters

[H1] Events
Join PacBio at upcoming events and conferences to hear from genomic experts and experience the latest innovations in long-read sequencing.
[H3] Expert Panel: Implementation of HiFi sequencing in clinical research laboratories
In this expert panel, hear how leading teams are evaluating HiFi WGS and PureTarget across rare disease, carrier screening, and broader human genomics workflows, with practical insight on assay consolidation, evaluation, adoption, and scale. Register now
[H3] Join us at ASM Microbe 2026 in June
June 4-7, 2026 – Visit us at booth #1325 to meet PacBio experts, see live demonstrations of our Vega and Revio sequencing systems, and learn how HiFi sequencing can empower your genetic testing workflows. Stay connected for the latest updates, sessions, and opportunities to connect with PacBio at ASM 2026. Access ASM 2026 content
[H3] Join us for Seq-it-Forward 5K to support rare disease research
June 27, 2026 – Join us for this family-friendly race supporting iHope’s mission to expand access to genetic testing and follow-up care for children and families affected by rare diseases, regardless of social status, income, or geographic location. Let’s come together to help families get the answers they deserve to create a path forward. Register now
[H2] Connect with the PacBio Community
Washington, DC
[H2] ASM Microbe 2026
June 04, 2026 - June 07, 2026 Science moves forward when we do. At ASM Microbe, the world’s premier gathering in the microbial sciences, you’ll explore the innovative ideas driving the next evolution of the field. Now organized as 3 independent, co-located meetings, ASM Microbe 2026 allows you to dive deep into your area of expertise and explore cutting-edge research across the broader microbial sciences, while expanding your network. More than a meeting, ASM Microbe is a community experience that connects scientists across disciplines and career stages through shared curiosity and discovery. Register Tokyo, Japan
[H2] The 8th Annual Meeting of the Japanese Association for Medical Artificial Intelligence (JMAI)
June 05, 2026 - June 06, 2026 The 8th Annual Meeting of the Japanese Association for Medical Artificial Intelligence (JMAI) will be held on June 5–6, 2026, at the Toranomon Hills Forum in Tokyo. PacBio and Tomy Digital Biology, will host an evening seminar on June 5, offering an opportunity to hear Prof. Allen Chan from The Chinese University of Hong Kong (CUHK) share his latest research and findings in deep learning using HiFi data. Event Site Gothenburg, Sweden
[H2] ESHG 2026
June 13, 2026 - June 16, 2026 The ESHG conference provides a platform for the dissemination of the most recent advancements in the field of human genetics, both scientifically and technologically. It also fosters the education of the upcoming generation of human geneticists. The conference is a unique opportunity to meet, share results, build scientific projects and collaborations and get inspired, whatever your professional background in human genetics. The meeting will also allow direct contact with exhibitors, presenting their new product portfolio and offering practical demonstrations. Event Site Webinar
[H2] Unlocking human microbiome clarity with PacBio HiFi sequencing
June 18, 2026 Thursday, June 18, 8:00 AM PDT | 11:00 AM EDT | 4:00 PM BST Join a live rebroadcast of this popular session on PacBio HiFi sequencing for human microbiome research, followed by a new live Q&A with PacBio microbial genomics expert Jeremy Wilkinson, PhD. In this session, you’ll hear how PacBio HiFi sequencing supports full-length 16S and shotgun metagenomics approaches for human microbiome research, helping researchers resolve species- and strain-level variation, uncover novel taxa, generate more complete MAGs, and improve pathway and functional annotation in complex microbial communities. Register Chiba, Japan
[H2] The 19th Annual Meeting of the Japanese Society for Epigenetics
June 25, 2026 - June 26, 2026 The 19th Annual Meeting of the Japanese Society for Epigenetics will be held on June 25–26, 2026, at the Chiba City Culture Center in Chiba, Japan. This meeting will bring together leading experts and researchers to share the latest discoveries and innovations in epigenetics. Be sure to visit our local distributor, Tomy Digital Biology, at their booth to learn how PacBio’s cutting-edge sequencing solutions can advance your epigenetics research. Event Site Del Mar, CA
[H2] Seq It Forward – Run for Rare 5K
June 27, 2026 June 27, 8:00 AM PST | Del Mar, CA Let’s come together for rare disease families! Join us for this family-friendly race supporting iHope’s mission to expand access to genetic testing and follow-up care for children and families affected by rare diseases, regardless of social status, income, or geographic location. Let’s come together to help families get the answers they deserve to create a path forward. Register Montreal, Canada
[H2] ASHG 2026
October 20, 2026 - October 24, 2026 Event Site CSHL campus, NY
[H2] Cold Spring Harbor Laboratory (CSHL) Microbiome Meeting
October 27, 2026 - October 31, 2026 The Cold Spring Harbor Laboratory (CSHL) Microbiome Meeting is a premier international conference bringing together leading researchers, clinicians, and industry scientists to explore cutting-edge developments in microbiome science. Held annually at the historic CSHL campus in New York, the meeting features a highly interactive program of keynote talks, short presentations, and poster sessions spanning microbial ecology, host–microbe interactions, computational analysis, and microbiome-based therapeutics. The conference is designed to foster cross-disciplinary exchange and collaboration, with participation from senior investigators alongside early-career scientists. Event Site Seattle, WA
[H2] AMP 2026
November 09, 2026 - November 13, 2026 Event Site
[H6] Events search
[H6] Event Type
[H6] Region
Clear filters

[H1] Featured Documentation
This Featured Documentation page provides the latest and most frequently used guides, protocols (procedures & checklists), and product information. For region-specific safety data sheets (SDS), search the SDS library. For product inserts and more see Other Documentation. May 26, 2026
[H3] Procedure & checklist — Preparing whole genome and metagenome sequencing libraries using SMRTbell prep kit 3.0
Procedure & checklist Sequel II system and Sequel IIe system, Revio system, Vega system Library preparation
[H2] Description
This procedure can be used to construct whole-genome sequencing (WGS) libraries from genomic and metagenomic DNA using the SMRTbell prep kit 3.0 for sequencing on PacBio HiFi systems. December 12, 2024
[H3] Operations guide — Revio system
Guide Revio system Sequencing
[H2] Description
This guide explains how to operate the Revio system. For information on instrument settings, sample setup, run design, user management, and Admin users, see the SMRT Link user guide. May 26, 2026
[H3] Guide & overview – Automated PureTarget kit 96 for the Hamilton NGS STAR MOA system
Guide & overview Revio system, Vega system Library preparation
[H2] Description
This procedure describes the automated workflow for generating PureTarget libraries using the PureTarget kit 96 on the Hamilton NGS STAR MOA system. The kit is designed for 96 samples per automated run. May 26, 2026
[H3] Guide & overview — Automated HiFi plex prep 96 for the Hamilton NGS STAR MOA system
Guide & overview Sequel II system and Sequel IIe system, Revio system, Vega system Library preparation, Automation
[H2] Description
This Guide & Overview describes the workflow for PacBio high-throughput semi-automated long read sample prep utilizing the HiFi plex prep 96 kit on the Hamilton NGS Microlab STAR MOA system. The HiFi plex prep kit 96 supports the multiplexing of 24 samples or more. May 26, 2026
[H3] Guide & overview — Automated-HiFi-prep-96-and-HiFi-ABC-for-the-Hamilton-NGS-STAR-MOA-system
Guide & overview Sequel II system and Sequel IIe system, Revio system, Vega system Library preparation, Automation
[H2] Description
This Guide & Overview describes the high throughput automated workflow for constructing whole genome sequencing (WGS) libraries from genomic DNA using the SRE HT kit, HiFi prep kit 96 and Revio polymerase kit 96 on the Hamilton NGS Microlab STAR MOA system. May 26, 2026
[H3] Procedure & checklist – Generating PureTarget libraries with PureTarget kit 24 manual protocol
Procedure & checklist Revio system, Vega system Library preparation
[H2] Description
This procedure describes the workflow for manually generating PureTarget libraries. May 26, 2026
[H3] Procedure & checklist – Generating PureTarget libraries with PureTarget kit 96 automation protocol
Procedure & checklist Revio system, Vega system Library preparation, Automation
[H2] Description
This procedure describes the workflow for generating PureTarget repeat expansion or carrier libraries. May 26, 2026
[H3] Procedure & checklist — Amplifying genomic DNA for SMRTbell library preparation and HiFi sequencing
Procedure & checklist Revio system, Vega system Sample preparation, DNA Shearing, Library preparation
[H2] Description
This procedure describes the workflow for constructing SMRTbell libraries with PCR amplification from as little as 1 ng of starting genomic DNA using the Ampli-Fi protocol. Sequencing libraries prepared with this procedure are compatible with PacBio Revio and Vega systems. May 26, 2026
[H3] Procedure & checklist — Preparing Iso-Seq libraries using SMRTbell prep kit 3.0
Procedure & checklist Sequel II system and Sequel IIe system, Revio system, Vega system Library preparation
[H2] Description
This procedure describes the workflow for constructing Iso-Seq libraries using SMRTbell prep kit 3.0 from RNA for sequencing on PacBio HiFi systems May 26, 2026
[H3] Procedure & checklist — Preparing Kinnex libraries from 16S rRNA amplicons
Procedure & checklist Sequel II system and Sequel IIe system, Revio system, Vega system Library preparation, Barcoding, Sequencing
[H2] Description
This procedure provides instructions for generating Kinnex libraries from full-length 16S amplicons for sequencing on PacBio HiFi systems. May 26, 2026
[H3] Procedure & checklist — Preparing Kinnex libraries using Kinnex single-cell RNA kit
Procedure & checklist Sequel II system and Sequel IIe system, Revio system, Vega system Library preparation
[H2] Description
This procedure describes the workflow for constructing single-cell Kinnex libraries from 10x Chromium 3’ or 5’ cDNA using the Kinnex single-cell RNA kit (103-072-200) for library prep and sequencing on PacBio HiFi systems. May 26, 2026
[H3] Procedure & checklist — Preparing Kinnex libraries using the Kinnex full-length RNA kit
Procedure & checklist Sequel II system and Sequel IIe system, Revio system, Vega system Library preparation
[H2] Description
This procedure describes the workflow for constructing Kinnex full-length RNA libraries from total RNA samples for sequencing on PacBio HiFi systems. May 26, 2026
[H3] Procedure & checklist — Preparing multiplexed AAV SMRTbell libraries using SMRTbell prep kit 3.0
Procedure & checklist Sequel II system and Sequel IIe system, Revio system, Vega system Library preparation
[H2] Description
This procedure can be used to prepare multiplexed AAV libraries for sequencing on the Sequel II and Sequel IIe systems. Multiplexing is achieved using SMRTbell barcoded adapters. May 26, 2026
[H3] Procedure & checklist — Preparing multiplexed amplicon libraries using SMRTbell prep kit 3.0
Procedure & checklist Sequel II system and Sequel IIe system, Revio system, Vega system Library preparation
[H2] Description
This procedure describes the workflow for constructing amplicon libraries using the SMRTbell prep kit 3.0 for sequencing on PacBio HiFi systems. Amplicons may be indexed during PCR, or during library preparation with SMRTbell indexed adapters. May 26, 2026
[H3] Procedure & checklist — Preparing multiplexed whole genome and amplicon libraries using the HiFi plex prep kit 96
Procedure & checklist Sequel II system and Sequel IIe system, Revio system, Vega system Library preparation
[H2] Description
This procedure describes the workflow for constructing multiplexed whole genome sequencing (WGS) and amplicon libraries using the HiFi plex prep kit 96 for sequencing on PacBio HiFi systems. The HiFi plex prep kit 96 supports the multiplexing of 24 samples or more.
[H6] Search
[H6] Filter by asset type
[H6] Filter by workflow step
[H6] Sequencing platforms
Clear filters